Integrated direct method with isomorphous replacement – second neighbourhood of quintets
نویسندگان
چکیده
منابع مشابه
The Sint~le Isomorphous Replacement Method
and refinement was usually complete after ten to fifteen cycles. The refined values of the parameters compared well with the Rossmann programme values, except for the effective atomic numbers, which were uniformly smaller than indicated by the Rossmann programme. The Hart hO1 values were used in the 2 /~. Fourier synthesis, although there may be some question remaining about the proper Z values...
متن کاملCorrelated phasing of multiple isomorphous replacement data.
Substantial highly correlated differences sometimes exist between a series of heavy-atom derivatives of a macromolecule and the native structure. Use of such a series of derivatives for phase determination by multiple isomorphous replacement (MIR) has been difficult because MIR analysis has treated errors as independent. A simple Bayesian approach has been used to derive probability distributio...
متن کاملMAD phasing: treatment of dispersive differences as isomorphous replacement information.
A framework analogous to that used for the analysis of data in the method of isomorphous replacement is applied to the analysis of multi-wavelength anomalous-diffraction (MAD) data. The present method is advantageous in that refinement of parameters describing the anomalously scattering atoms in the structure can be carried out using nearly all the data, and in that phase information can be rea...
متن کاملIsomorphous Replacement : Effects of Errors on the Phase Probability Distribution
A simple framework for the analysis of the effects of errors in the method of isomorphous replacement is developed. This framework is used to derive phase probability distributions similar to those given by Blow & Crick [Acta Cryst. present analysis suggests an improved means of calculating the mean-square 'lack-of-closure' residuals and shows that they differ by a factor of two for centric and...
متن کاملAn isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorph...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1996
ISSN: 0108-7673
DOI: 10.1107/s0108767396096912